Description

AneuQuick™ Max Plus v2.2

AneuQuick™ Max Plus v2.2 is a comprehensive QF-PCR (Quantitative Fluorescent PCR) kit developed for the rapid detection of chromosomal aneuploidies involving chromosomes 13, 18, 21, X, and Y, while simultaneously screening for Spinal Muscular Atrophy (SMA) through the detection of SMN1 exon 7 deletion/conversion and SMN2.

The assay combines 35 carefully selected genetic markers in a single-tube multiplex PCR reaction, allowing laboratories to detect the most common fetal chromosomal abnormalities—including Down syndrome (Trisomy 21), Edwards syndrome (Trisomy 18), Patau syndrome (Trisomy 13), Turner syndrome (Monosomy X), Klinefelter syndrome (47,XXY), Triple X syndrome (47,XXX) and other sex chromosome aneuploidies—while assessing the SMN1/SMN2 status in the same workflow.

AneuQuick™ Max Plus v2.2 is compatible with DNA extracted from peripheral blood, amniotic fluid, chorionic villus samples (CVS), tissue specimens, cell-free DNA from amniotic fluid, and KBC DBC™ DNA Storage Cards. By combining an expanded STR panel, Segmental Duplication markers, and dedicated SMA markers, the kit provides a practical and efficient solution for molecular genetics laboratories and prenatal diagnostic centers seeking comprehensive genetic screening in a single assay.

Features

Key Features & Benefits

Simultaneous detection of aneuploidies involving chromosomes 13, 18, 21, X, and Y, together with SMN1 exon 7 deletion/conversion
Detects common chromosomal disorders including Down, Edwards, Patau, Turner, Klinefelter, Triple X, and other sex chromosome abnormalities
Based on QF-PCR technology for rapid prenatal testing with a streamlined workflow
Amplifies 35 genetic markers in a single-tube multiplex PCR reaction
Includes three Segmental Duplication markers (7/X, 11/X, and 18/X) for improved assessment of X chromosome copy number and more confident detection of Turner syndrome
Features five additional STR markers compared with AneuQuick™ Max v2.2 for enhanced chromosome coverage and result interpretation
Enables simultaneous evaluation of SMN1 and SMN2, eliminating the need for a separate SMA screening assay
Compatible with multiple prenatal and clinical DNA sample types
Optimized for 6-dye fragment analysis
Delivers reliable, reproducible performance for molecular genetics, prenatal diagnosis, and research laboratories

مارکرها

Markers

AneuQuick™ Max Plus v2.2 incorporates 35 genetic markers distributed across chromosomes 13, 18, 21, X, and Y. The panel combines highly informative STR markers, Segmental Duplication markers, and dedicated SMN1/SMN2 markers, providing comprehensive analysis of both chromosomal aneuploidies and SMA-associated variants within a single assay.

Figure 1. Distribution of AneuQuick™ Max Plus v2.2 markers with the KBC600™ Size Standard.

To improve the accuracy of X chromosome analysis, the kit includes 7/X, 11/X, and 18/X Segmental Duplication markers, which help distinguish Turner syndrome (Monosomy X) from STR homozygosity and provide additional confidence when evaluating X chromosome copy number.

In addition, dedicated SMN1 and SMN2 markers enable simultaneous assessment of SMN1 exon 7 deletion or conversion, extending the clinical value of the assay beyond conventional QF-PCR aneuploidy testing.

علاوه بر این، وجود دو مارکر اختصاصی SMN1 و SMN2 امکان بررسی هم‌زمان حذف یا تبدیل اگزون 7 ژن SMN1 را فراهم کرده و ارزش تشخیصی این کیت را در مقایسه با کیت‌های متداول QF-PCR افزایش می‌دهد.

Comparison Table

Product Family Comparison

The AneuQuick™ product family offers a range of QF-PCR kits designed for rapid prenatal aneuploidy screening and tailored to different laboratory requirements. Depending on the application, laboratories can choose from standard panels, expanded marker panels, or advanced kits with integrated SMA detection.

The comparison table below summarizes the key specifications of each AneuQuick™ kit, including marker count, chromosome coverage, Segmental Duplication markers, SMA detection capability, fluorescence chemistry, and instrument compatibility, making it easier to identify the most suitable solution for your workflow.

Feature	AneuQuick v4.2	AneuQuick Plus v2.2	AneuQuick Extra v2.2	AneuQuick Max v2.2	AneuQuick Extra Plus v2.2	AneuQuick Max Plus v2.2
Total Markers	27	29	33	33	35	35
X markers	7	7	8	8	8	8
X/Y markers	2	2	2	2	2	2
Chr. 21 markers	6	6	8	8	8	8
Chr. 18 markers	5	5	8	7	8	7
Chr. 13 markers	5	5	6	7	6	7
Segmental Duplication	2	2	8	3	8	3
SMA Detection	No	✓	No	No	✓	✓
5- or 6-dye system	5	5	6	6	6	6
Single tube multiplex	✓	✓	✓	✓	✓	✓
Compatible with	Spectrum Compact CE from Promega, 3130/xl, 3500/xL and SeqStudio platforms	Spectrum Compact CE from Promega, 3130/xl, 3500/xL and SeqStudio platforms	Spectrum Compact CE from Promega, 3500/xL and SeqStudio platforms	Spectrum Compact CE from Promega, 3500/xL and SeqStudio platforms	Spectrum Compact CE from Promega, 3500/xL and SeqStudio platforms	Spectrum Compact CE from Promega, 3500/xL and SeqStudio platforms