Introduction to MRC Holland
MRC Holland company, located in Amsterdam, is a biological company in the field of producing enzymes and molecular markers. Since 2002, it has also been providing MLPA technology.
MLPA assay is used worldwide, and MLPA technology is considered the gold standard for determining DNA sequence copy numbers. In addition to MLPA products, MRC Holland also develops, produces, and sells Melt Assay products and digitalMLPA probemixes.
Kawsar Biotech Company is the official representative of MRC Holland in the region. Dear customers can contact us to benefit from the company’s MLPA services and purchase MLPA kits. Additionally, genomic services based on MLPA are available, allowing you to send samples to Kawsar Biotech Company for the necessary tests to be performed by our experts.
What is MLPA?
Copy number variations (CNVs) are the most significant cause of genetic diversity in human DNA and play a role in numerous human genetic disorders. MLPA is a semi-quantitative and non-automated technique used to determine the relative copy number of up to 60 DNA sequences in a single reaction, based on multiplex PCR.
The principle of MLPA involves amplifying and replicating approximately 60 probe sequences, each targeting specific DNA sequences of around 60 base pairs in length.
Figure 1: MLPA reaction results in the production of a set of unique PCR amplicons ranging in length from 64 to 500 base pairs, which are separated by capillary electrophoresis. After initial denaturation of the DNA template, a mixture of MLPA probes is added to it. In general, each MLPA probe consists of two oligonucleotides that must anneal adjacent to each other on the target sequences to form a unique probe.
During the subsequent PCR reaction, all the probes generated during ligation are simultaneously amplified using the same pair of PCR primers. As a result, a set of unique PCR amplicons is created. One PCR primer is fluorescently labeled, which allows the amplified PCR products to be visualized during separation in the genetic analyzer device. This process creates a specific electropherogram, and the data analysis is performed using Coffalyser.Net software.
