Chimerism DTekt™ Kit v1.2

The bone marrow transplant (BMT) is a specialized treatment for more than 40 different diseases, including leukemias, thalassemia, Sickle Cell disease, etc. It involves the extraction of cells commonly found in the bone marrow (stem cells), which are then filtered and transplanted to either the donor or another individual who has matching HLA. One of the essential aspects of bone marrow transplant is the replacement of the donor’s genetic profile in the recipient, as the successful transplant depends on the absence of the donor’s cells in the recipient. The recipient has specific genotype cells, and when the transplant occurs, the recipient is considered to have genetic chimerism, indicating the presence of two genotypes and essentially having two different sets of DNA.

The Chimerism DTekt™ Kit v1.2 allows for the assessment of the gradual replacement of the recipient’s genetic profile with that of the donor. This particular test is conducted on individuals who have undergone hematopoietic stem cell transplantation, aiming to ascertain the proportion of genetic material contributed by each individual and identify the dominant genetic profile.

This kit utilizes a total of 17 markers, comprising of 16 STR markers (short tandem repeat sequences consisting of 3 to 7 base pairs) with a high level of heterozygosity, along with one marker specifically designed for sex determination. The amplification of all 17 markers is achieved through the Multiplex PCR method, and the subsequent analysis of the results is conducted using capillary electrophoresis.

The Chimerism DTekt™ Kit v1.2 is a multiplex PCR kit. This kit utilizes fluorescence and consists of 16 STR markers, including CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, D19S433, D2S1338, SE33, and AMXY (sex markers). With this kit, users can obtain a unique profile for each individual. These profiles resemble those used in forensic genetics and human identification. In cases where two or more DNA samples are mixed together, multiple peaks will be observed for each marker or locus. In chimerism tests conducted after bone marrow transplantation (BMT), physicians aim to determine whether there is a single DNA profile or if there are two profiles present – one resembling the donor’s and the other resembling the recipient’s, though with varying peak heights.