PBS 1X

Phosphate-buffered saline (PBS) is a versatile buffer solution widely used in biological research, particularly for cell culture and molecular biology applications. With a pH of approximately 7.4, it closely resembles the osmolarity and ion concentrations of the human body, making it isotonic and non-toxic to cells. PBS is composed of water-based salts, including disodium hydrogen phosphate and sodium chloride, and may also contain potassium chloride and potassium dihydrogen phosphate in certain formulations. The primary function of PBS is to maintain a stable pH environment for experiments involving cells or biological tissues. It’s commonly used for rinsing cells, diluting substances, and as a transport medium for tissue samples. The preparation of PBS can vary, but typically involves dissolving the appropriate salts in distilled water and adjusting the pH if necessary. PBS solutions can be sterilized through autoclaving or filtration and are available in different concentrations for various research needs. Its non-toxic nature and ability to mimic physiological conditions make PBS an essential tool in the life sciences.