DMEM Low +Glutamax

The cell culture medium of Kawsar Biotech Company is generally a suitable source of energy and nutrients that leads to the growth of many human cells and cell lines and regulates the cell cycle. Common components of the culture medium usually consist of amino acids, vitamins, minerals, glucose, and serum.

There are different types of cell culture media that depend on the type of cell, and each of the Stem Cells, Primary Cells, and Cell Lines, whether adherent or suspended, usually require different types of culture media, either normal or containing more nutrients for optimal growth.

DMEM-Low+Glutamax is a specialized variant of Dulbecco’s Modified Eagle Medium (DMEM) that supports the growth of mammalian cells in vitro. It is formulated with a lower glucose concentration, typically 1 g/L, which is beneficial for cells that require less energy or are sensitive to high glucose levels. The addition of DMEM-Low+Glutamax, a dipeptide substitute for L-glutamine, enhances cell viability and growth by preventing the spontaneous degradation of L-glutamine into ammonia, which can be detrimental to cell health.

This medium is ideal for researchers who need a stable and long-lasting source of L-glutamine without the toxic byproducts of its breakdown. DMEM-Low+Glutamax is often used with various cell types, including primary cells and sensitive cell lines, due to its reduced osmolarity compared to high-glucose DMEM. It is also supplemented with sodium pyruvate, another key energy source for cells. The medium is buffered with sodium bicarbonate and requires a CO2 environment to maintain physiological pH. It is commonly supplemented with fetal bovine serum (FBS) to provide additional growth factors and proteins necessary for cell proliferation and maintenance.