KBC Hi-Fid™ DNA Polymerase
Cat. No: KBC-520103KBC Hi-Fid™ DNA Polymerase (High Fidelity) is a recombinant enzyme capable of amplifying fragments up to 4 Kb with high accuracy. This enzyme is commonly used in molecular biology and genetics research. It is known for its high fidelity, meaning it has a low error rate when replicating DNA compared to other DNA polymerases. KBC Hi-Fid™ is often used in PCR (polymerase chain reaction) to amplify DNA with high accuracy, making it particularly useful for applications where maintaining the integrity of the DNA sequence is crucial, such as sequencing and cloning.
This polymerase is derived from a thermophilic (heat-loving) microorganism, which allows it to function at high temperatures, making it suitable for PCR. Additionally, HiFi DNA Polymerase possesses a 3′ to 5′ exonuclease activity, which enables it to proofread newly synthesized DNA, further contributing to its high fidelity.
Overall, HiFi DNA Polymerase is a valuable tool in genetics and molecular biology research due to its ability to accurately replicate DNA sequences, making it an essential component in various experimental techniques.
- Features:
- It has maximum power with high functional accuracy and low percentage of error in the amplification process
- Ability to polymerize DNA fragments up to 4 Kb
- HiFid 10X buffer suitable for this enzyme due to having a standard pH, suitable salt concentration and long-term high temperature tolerance
- Without the need for buffers and auxiliary solutions in PCR, able to replicate DNA with 65% GC percentage
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