PAH Gene
confirm the correct functioning of primers and other components of PCR and to confirm the efficiency of the desired sequence amplification step, control DNA with a specific profile can be used as a known indicator with a certain genotype. In fact, control DNA can be used as a reference standard. It was used to measure the usual quality control of the examined sample.
Considering the importance of prenatal diagnosis in molecular genetics and the identification of pathogenic mutations, the use of control DNA related to the desired disease, both in terms of directly examining the pathogenic genotype and in terms of examining the SNP pattern in the genes investigated in the method RFLP is important.
All provided DNA controls were confirmed by sequencing or MLPA with an optical absorbance (OD) of 1.7-2.
- Presentation in a volume of 50 microliters (300ng/μL)
- Used in PCR for various purposes
- To check the presence or absence of PCR product
- Examining the quality of DNA in terms of structure and degradation rate
- Ideal for most standard genetic research applications
- Avoid errors
- Eligible for genetic birth certificate
- having normal, heterozygous or homozygous genotypes
- Reliable amplification of long DNA fragments
- Southern hybridization analysis
- Genomic library construction
- This mutation involves replacing guanine (G) with thymine (T) in intron 4 at position 5.
- This mutation involves a substitution in which a guanine (G) is replaced by a cytosine (C) at the +1 position of the splice donor site.
- This mutation involves replacing a cytosine (C) with a thymine (T) in the codon.
- The “C.592-613 del” mutation refers to a deletion mutation in the genetic sequence from nucleotide position 592 to 613.
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