KBC Enhancer Buffer

The enhancer buffer is a component used in PCR (Polymerase Chain Reaction) mixes to optimize the reaction, especially for DNA sequences rich in GC content. It is designed to improve the specificity of the PCR product by increasing the annealing temperature and enhancing the performance of the DNA polymerase enzyme.

At a concentration of 5 Molar, the enhancer buffer increases the specificity of the PCR product and reduces the DNA melting temperature (TM), thereby boosting the efficiency of the DNA polymerase enzyme. Additionally, the enhancer buffer stabilizes the AT-rich regions in the major groove of the DNA double helix, promoting the proximity of GC and AT base pairs to each other. This equalization of the melting temperatures of AT and GC-rich regions ultimately reduces the overall melting temperature of the DNA.

Moreover, the enhancer buffer aids in the function of heat-resistant polymerases during the amplification process, reducing pauses caused by secondary structure and allowing for continuous polymerization.