KBC Master Mix

The master mix for PCR (polymerase chain reaction) is a solution containing all the components needed for the PCR reaction, except for the template DNA and the primers. The master mix typically includes buffer, dNTPs (deoxynucleotide triphosphates), DNA polymerase, and magnesium chloride.

The buffer provides the optimal pH and ionic strength for the PCR reaction, while the dNTPs are the building blocks (nucleotides) used by the DNA polymerase to synthesize new DNA strands. The DNA polymerase is the enzyme responsible for replicating the DNA, and magnesium chloride is often included to help optimize the enzyme’s activity.

By preparing a master mix, it’s possible to ensure that all the necessary components are present in the correct proportions, which can help to minimize pipetting errors and improve the consistency of the PCR reactions. This is especially important when setting up multiple reactions, as it helps to reduce variability between them.

Storage Conditions: from -25° C to -15° C

Kit Contents: KBC Master Mix