KBC Taq DNA Polymerase

The KBC Taq DNA Polymerase enzyme is a heat-resistant enzyme isolated from the bacterium Thermus aquaticus, which, in the presence of dNTP, suitable buffer, and primer, under stable conditions, synthesizes the second DNA strand from the template strand.

This enzyme has the ability to synthesize DNA in the 5′ → 3′ polymerase and 5′ → 3′ exonuclease directions, but is known for its lack of 3′ to 5′ exonuclease proofreading activity, which results in a higher error rate and makes it prone to introducing mutations during DNA synthesis.

With its polymerase activity, KBC Taq DNA Polymerase amplifies DNA fragments up to 2kb in length using the PCR method, which is suitable for use in various PCR-based techniques such as QF, MLPA, and Cycle Sequencing, and has found general application.

In addition to its polymerase activity, it also has Terminal deoxynucleotidyl transferase activity, which leads to the addition of an adenine at the 3′ end of the PCR product. This enzyme’s feature makes it suitable for use in amplifying fragments for cloning in the TA vector.