DMEMF-12 +Glutamax

The cell culture medium of Kawsar Biotech Company is generally a suitable source of energy and nutrients that leads to the growth of many human cells and cell lines and regulates the cell cycle. Common components of the culture medium usually consist of amino acids, vitamins, minerals, glucose, and serum.

There are different types of cell culture media that depend on the type of cell, and each of the Stem Cells, Primary Cells, and Cell Lines, whether adherent or suspended, usually require different types of culture media, either normal or containing more nutrients for optimal growth.

DMEM-F12+Glutamax is a specialized cell culture medium designed to support the growth of mammalian cells. It is a modification of the classic DMEM-F12, a 1:1 mixture of Dulbecco’s Modified Eagle Medium and Ham’s F-12, which is known for its high concentrations of glucose, amino acids, and vitamins. The addition of Glutamax, a stabilized form of L-glutamine, enhances this medium by preventing the degradation of L-glutamine into ammonia, which can be toxic to cells. This feature improves cell viability and growth, making DMEM-F12+Glutamax an excellent choice for long-term cell culture applications.

The medium is free of proteins, lipids, or growth factors, so it typically requires supplementation with 10% Fetal Bovine Serum (FBS). It uses a sodium bicarbonate buffer system and needs a 5-10% CO2 environment to maintain physiological pH. DMEM-F12+Glutamax is suitable for a variety of cell types, including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. It is available in liquid form, is sterile-filtered, and should be stored at 2-8°C, protected from light. The shelf life is 12 months from the date of manufacture, and it’s important to note that this medium is for research use or further manufacturing, not for diagnostic use or direct administration into humans or animals.