KBC’s culture mediums are generally suitable source of energy and nutrients that leads to the growth of all types of cells including human cell lines and regulates the cell cycle. The common components of the culture medium usually consist of amino acids, vitamins, mineral salts, glucose, and serum.
There are different types of cell culture media. The use of each type of media depends on the type of cell, and each of the Stem Cells, Primary Cells, and Cell Lines requires different types of culture media, either adhesive or suspension, usually containing additional nutrients for optimal growth.
Dulbecco’s Modified Eagle Medium (DMEM) is a popular basal medium for mammalian cell culture. It has a high concentration of amino acids and vitamins and is available in various glucose concentrations and with optional sodium pyruvate. DMEM supports the growth of a wide range of cells, including fibroblasts, neurons, and several cell lines. This medium uses a sodium bicarbonate buffer system and typically requires fetal bovine serum (FBS) to create a complete growth environment. This medium is essential for applications such as protein production, cell differentiation, and drug development. Different formulations of DMEM are available to meet specific research needs, and it is crucial to supplement it appropriately for optimal cell culture results.
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