AZFCheck Plus Kit v3.2

Y Chromosome Microdeletion Detection and Klinefelter Syndrome Analysis Kit – AZFCheck™ Plus Kit v3.2

AZFCheck™ Plus Kit v3.2, developed by Kawsar Biotech Company, is a molecular genetics kit designed for the detection of Y chromosome microdeletions associated with male infertility. The kit enables the analysis of microdeletions in the Azoospermia Factor (AZF) regions, including AZFa, AZFb, and AZFc, and can also be used to investigate genetic profiles associated with Klinefelter syndrome.

The kit features 36 genetic markers, comprising 26 Sequence-Tagged Sites (STS), three Segmental Duplication (SD) markers, and seven X-chromosome Short Tandem Repeat (STR) markers. These markers are amplified simultaneously in a single PCR reaction and analyzed using five-dye fluorescent fragment analysis by capillary electrophoresis. The markers have been selected and designed with reference to the EAA/EMQN 2023 guidelines for Y chromosome microdeletion analysis and the assessment of genetic markers relevant to sex chromosome abnormalities.

AZFCheck™ Plus Kit v3.2 is compatible with Applied Biosystems genetic analyzers, including ABI 3130/3130xl, ABI 3500/3500xL, and SeqStudio platforms, as well as the Compact Spectrum CE System from Promega. The resulting fragment analysis data can be evaluated using GeneMapper software. Suitable sample materials include extracted DNA from blood, blood samples stored on DNA Banking Cards (DBC)

, and diluted blood prepared using appropriate Kawsar Biotech Company blood dilution buffers.

AZFCheck™ Plus Kit v3.2 provides a multiplex molecular approach for investigating Y chromosome microdeletions associated with male infertility and genetic markers relevant to Klinefelter syndrome. With simultaneous amplification of 36 markers and five-dye capillary electrophoresis, the kit is designed for use in specialized genetics laboratories and molecular research applications.