AneuQuick™ v4.2

The AneuQuick™ Kit v4.2 is a quantitative fluorescence polymerase chain reaction (QF-PCR) molecular diagnostic kit used for prenatal screening and rapid detection of common chromosomal numerical abnormalities.

When the number of chromosomes is not a multiple of 23 (for example, instead of 46 chromosomes, a person has 47 chromosomes), this condition is referred to as chromosomal numerical abnormalities or aneuploidy. The most common aneuploidies are chromosomes 21, and the sex chromosomes X and Y. Other detectable aneuploidies with this kit include trisomies of chromosomes 13 and 18. Chromosomal abnormalities can also be investigated through cytogenetic methods and performing karyotyping, which typically requires at least two weeks for fetal cells to grow and the potential aneuploidy status to be assessed. However, recently, the new molecular method QF-PCR has become significantly prevalent as it can be performed in a shorter time and offers cost-effectiveness and very high accuracy. This method enables precise results within a maximum of 5 hours.

The AneuQuick™ v3.2 kit contains 26 STR markers from chromosomes 13, 18, 21, X, and Y (21 STR markers with high heterozygosity and 4 markers for gender determination and sex chromosome abnormalities). By adding the marker 11X to the KBC AneuQuick™ v3.2 kit, it is released as AneuQuick™ v4.2. The 7X and 11X marker are also segmental duplication markers included in the kit to distinguish X chromosome monosomy from homozygosity and to detect Turner syndrome. These STR markers in both versions are simultaneously examined using quantitative fluorescence polymerase chain reaction (QF-PCR). Therefore, the AneuQuick™ v3.2 & v4.2 kits can be used in the following cases:

• Diagnosis of Down syndrome (Trisomy 21)
• Diagnosis of Edwards syndrome (Trisomy 18)
• Diagnosis of Patau syndrome (Trisomy 13)
• Diagnosis of Turner syndrome (X, 45)
• Diagnosis of Klinefelter syndrome (XXY, 47)
• Diagnosis of Jacob syndrome (XYY, 47)
• Diagnosis of Triple X syndrome (XXX, 47)
• Other aneuploidies related to chromosomes X and Y
• Diagnosis of triploidy and tetraploidy
• Gender determination

It is worth mentioning that QF-PCR is a diagnostic method, while NIPT is a screening method. Therefore, the AneuQuick™ v4.2 kit is a suitable alternative for confirming the test after the NIPT results have been positive.