Chimerism DTekt™ Kit v1.2 Allelic Ladder

The Chimerism DTekt™ v1.2 Allelic Ladder is a dedicated allelic reference for analyzing results from the Chimerism DTekt™ v1.2 kit, which is used to monitor the gradual replacement of the recipient’s genetic profile with that of the donor following bone marrow transplant (BMT). This product contains a selected set of alleles for the kit’s STR markers, and by providing the reference positions of the alleles, it helps ensure accurate allele identification and designation (Allele Calling) in analysis software.

This Allelic Ladder includes selected alleles for the markers AMXY, D7S820, D21S11, VWA, D3S1358, D19S433, D8S1179, D13S317, CSF1PO, D2S1338, TH01, D5S818, TPOX, SE33, D16S539, D18S51, and FGA.

For analysis, the Allelic Ladder is prepared just like a PCR product and is electrophoresed alongside the samples on a Genetic Analyzer. After electrophoresis, the Ladder file is imported together with the sample files into an analysis software such as GeneMapper™ ID/ID-X. Using the Allelic Ladder that matches the corresponding kit and product version is essential for accurate Allele Calling and correct result interpretation.

To view the complete list of alleles, the allelic range of each marker, and the reference electropherogram, please refer to the User Manual of the Chimerism DTekt™ v1.2 kit in the product documentation section.