The KBC HS Taq DNA Polymerase is an engineered heat-resistant enzyme with the capability of synthesizing DNA in the 5′→3′ polymerase direction and exhibiting 5´ flap endonuclease activity. The Hot Start Taq DNA Polymerase is a chemically modified enzyme that remains inactive at room temperature and requires an increase in temperature for activation.
Unlike conventional enzymes, the HS Taq DNA Polymerase undergoes a distinctive chemical alteration. This modification renders it inactive at room temperature, requiring a rise in temperature for activation. Once activated, its potent polymerase activity drives the amplification of DNA fragments up to 3 kb in length through the PCR method, ensuring reliable and efficient replication.
Adding to its enzyme power, the special PT 10X buffer boosts the HS Taq DNA Polymerase even more. It’s important to note that this special buffer doesn’t have MgCl2 in it. Instead, the MgCl2 solution is given separately to make sure the PCR reactions work perfectly.
This enzyme, with its polymerase activity, amplifies DNA fragments up to 3 kb in length using the PCR method.
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