Description

AneuQuick™ Plus v2.2

AneuQuick™ Plus v2.2 is a molecular diagnostic kit based on quantitative fluorescence polymerase chain reaction (QF-PCR), used for prenatal screening and rapid diagnosis of common chromosomal numerical abnormalities 13, 18, 21, X, and Y, as well as deletions, point mutations, or gene conversion in exon 7 of the SMN1 and SMN2 genes in spinal muscular atrophy (SMA). In fact, this kit can detect not only chromosomal abnormalities such as Down syndrome (or trisomy 21), Edwards syndrome (or trisomy 18), Patau syndrome (or trisomy 13), Klinefelter syndrome (XXY), Triple X syndrome (XXX), monosomy X (Turner syndrome), and other sex chromosome abnormalities but also diagnose 5q SMA.

The AneuQuick™ Plus v2.2 kit contains 28 markers (22 STR markers with high heterozygosity, 4 markers for gender determination and sex chromosome abnormalities, and two single nucleotide polymorphism (SNP) polymorphic markers for the SMN1 and SMN2 genes). These SNP markers are added to the KBC AneuQuick™ v4.2 kit for detecting deletions and mutations in the SMN1 and SMN2 genes. Additionally, the 7X marker is a segmental duplication marker included in the kit to differentiate X chromosome monosomy from homozygosity, thus diagnosing Turner syndrome.

It’s worth mentioning that QF-PCR is a diagnostic method, while NIPT is a screening method. Therefore, the AneuQuick™ Plus v2.2 kit serves as a suitable alternative for confirming the test after positive NIPT results.

Features and Benefits

- Analysis of 28 markers in a single reaction
- Use of STR markers with high heterozygosity
- Rapid diagnostic follow-up for NIPT
- Optimized for working with various human DNA sources
- Easy to useFeatures and Benefits
This kit is compatible with DNA samples extracted from blood, amniotic fluid (AF), chorionic villus sampling (CVS), and DBC cards (DNA storage cards by Kowsar Biotechnology). It is compatible with blood lysis buffer (BLB), amniotic fluid lysis buffer (AFL), and chorionic villus lysis buffer (CVL).

For further information on the product, its usage, and result analysis, please refer to the user manual.

AneuQuick™ Plus v1.2 Kit Markers

Figure 1. Map of AneuQuick Plus v2.2 kit marker

Comparison Table

Table 1. indicates the number of markers in each kit. There are 26 markers in the AneuQuick Kit v4.2, 28 in the AneuQuick™ Plus Kit v2.2, 32 in the AneuQuick™ Extra Kit v2.2, and 34 in the AneuQuick™ Extra Plus Kit v2.2. Additionally, there are 8 segmental duplications markers in both the AneuQuick™ Extra Kit v2.2 and AneuQuick™ Extra Plus Kit v2.2. The markers in these kits, amplified through multiplex fluorescent-based PCR, provide higher detection capability to medical genetic specialists. The inclusion of creative SMA detection (by adding exon 7 of the SMN1 and SMN2 genes in the AneuQuick™ Plus Kit v2.2 and AneuQuick™ Extra Plus Kit v2.2) is a notable advantage for any laboratory. It enables simultaneous detection of cases where the fetal sample appears normal in terms of aneuploidy but is affected by a severe and costly disease like SMA.

Table 1. Comparison Table

Feature	AneuQuick v4.2	AneuQuick Plus v2.2	AneuQuick Extra v2.2	AneuQuick Max v2.2	AneuQuick Extra Plus v2.2	AneuQuick Max Plus v2.2
Total Markers	27	29	33	33	35	35
X markers	7	7	8	8	8	8
X/Y markers	2	2	2	2	2	2
Chr. 21 markers	6	6	8	8	8	8
Chr. 18 markers	5	5	8	7	8	7
Chr. 13 markers	5	5	6	7	6	7
Segmental Duplication	2	2	8	3	8	3
SMA Detection	No	✓	No	No	✓	✓
5- or 6-dye system	5	5	6	6	6	6
Single tube multiplex	✓	✓	✓	✓	✓	✓
Compatible with	Spectrum Compact CE from Promega, 3130/xl, 3500/xL and SeqStudio platforms	Spectrum Compact CE from Promega, 3130/xl, 3500/xL and SeqStudio platforms	Spectrum Compact CE from Promega, 3500/xL and SeqStudio platforms	Spectrum Compact CE from Promega, 3500/xL and SeqStudio platforms	Spectrum Compact CE from Promega, 3500/xL and SeqStudio platforms	Spectrum Compact CE from Promega, 3500/xL and SeqStudio platforms

Compatibility

This kit is compatible with 5-color capillary electrophoresis systems such as ABI PRISM 3130/3130xl/3500/3500xL Genetic Analyzer with capillary sizes of 30, 50, or 80 centimeters.

Documents

User Manual