Proteinase K 5mg/1ml

KBC Proteinase K, a serine protease, plays a role in protein digestion and nucleic acid decontamination processes. Its enzymatic activity involves cleaving peptide bonds adjacent to the carboxylic group of both aliphatic and aromatic amino acids. When introduced into nucleic acid preparations, Proteinase K serves to inactivate nucleases that may otherwise degrade DNA or RNA during extraction and purification procedures.

This versatile enzyme boasts remarkable stability and efficacy, remaining active even at room temperature. Unlike many other enzymes, KBC Proteinase K withstands the presence of chelating agents like EDTA and denaturants such as SDS, retaining its enzymatic activity under diverse conditions. Moreover, Proteinase K exhibits robust activity across a broad pH range, from 4 to 12.5, enhancing its utility in various biochemical applications.

Proteinase K belongs to the family of serine proteases associated with Subtilisin, and its activity can be effectively inhibited by phenylmethylsulfonyl fluoride (PMSF).