KBC AFL Buffer is a highly efficient buffer designed for lysing amniotic fluid samples and cultured fetal cells. It can be directly used in various PCR applications such as Multiplex PCR and STR-based kits for identity determination offered by Kawsar Biotech Company. The use of AFL buffer offers numerous advantages, including the elimination of the need for a specific method, significant savings in time and cost, and the absence of a purification process. Additionally, it requires minimal consumables, leading to cost savings, and reduces the risk of contamination during the testing process.
The required amount of amniotic fluid for direct use with AFL depends on the gestational age of the fetus. A higher number of cells in the sample will require a higher amount of AFL Buffer for direct use.
Furthermore, it is recommended to store any remaining lysed sample residue at a temperature of 20°C to maintain its stability. While the use of fresh samples is advised for optimal results, the preserved lysed sample can be utilized if necessary. This buffer provides a convenient and cost-effective solution for laboratories conducting molecular testing on amniotic fluid samples and cultured fetal cells, offering reliability and efficiency in genetic analysis.