YFiling™ (29-Plex) Kit v2.2

The KBC human identification profiling kits are used in various fields such as human identification, forensic medicine, parentage testing, kinship analysis, research, and so on.

Human identification profiling kits have been developed worldwide due to their extensive application in forensic genetics. These kits can be used to search databases and have high heterozygosity for most populations. Therefore, these kits can be used for various types of human identification, parentage testing, forensic medicine, and kinship studies. KBC HID kits are designed based on STR markers recommended by the European Network of Forensic Science Institutes (ENFSI), the National Institute of Standards and Technology (NIST), the International Society for Forensic Genetics (ISFG), and the Combined DNA Index System (CODIS).

Kawsar Biotech Company offers several HID kits for various purposes. These kits include:

• IRFiling™ kit (with 17 markers in a 5-color system) is a preferred kit for most forensic medical cases. According to CODIS, a minimum of 13 markers is recommended for identity determination, and this kit uses 17 markers.
• IRFiling™ Globe (with 27 markers in a 6-color system) is currently used more for forensic medical purposes worldwide, especially when the FBI introduced new CODIS markers in 2016.
• The X chromosome-related kit called XFiling™ with 17 markers, 15 of which are specific to the X chromosome, operates in a 5-color system.
• The Y chromosome-related kit named YFiling™ (29-Plex) with 30 markers, also in a 5-color system, can be used for criminal investigations as well as male kinship analysis.

A comparison table has been prepared for selecting the appropriate human profiling kit for forensic medical purposes and identification in line with the goals of our dear customers.

DNA contains unique sequences of short tandem repeats (STRs) consisting of 3 to 7 base pairs scattered throughout the genome, which can be used for individual identification in forensic medicine. Since the exact number of repeats at each specific location varies among different individuals, forensic medicine can utilize these markers for identification purposes. Furthermore, each individual has two sets of STRs inherited from both parents, and each of these sets can have a different number of repeats at the same locus. In fact, a DNA profile is recognized as a DNA fingerprint that distinguishes individuals from each other and is only similar in identical twins.

The YFiling™ (29-Plex) Kit v2.2 determines male relatedness through Y-chromosome profiling. In fact, the profiling is done by examining the short tandem repeat sequences of the Y chromosome, or Y-STRs. In forensic genetics, it is used for human identification and investigating kinship in male human samples. This kit is based on 30 markers, including 29 high heterozygosity STR markers. In addition to the 29 markers, a gender determination marker is added to the kit to detect sample contamination by another individual. One of the advantages of this kit compared to foreign equivalents is its ability to examine a greater number of markers. Furthermore, this kit can be read on 5-dye system devices such as the ABI Prism 3130/xl and 3500/xL Genetic Analyzers, while foreign kits require a 6-dye system for examining 25 loci, such as the ABI Prism 3500/xL Genetic Analyzer. All 30 markers are amplified by the Multiplex PCR method, and the results are determined using capillary electrophoresis