IRFiling™ Kit v2.2

The KBC human identification profiling kits are used in various fields such as human identification, forensic medicine, parentage testing, kinship analysis, research, and so on.

Human identification profiling kits have been developed worldwide due to their extensive application in forensic genetics. These kits can be used to search databases and have high heterozygosity for most populations. Therefore, these kits can be used for various types of human identification, parentage testing, forensic medicine, and kinship studies. KBC HID kits are designed based on STR markers recommended by the European Network of Forensic Science Institutes (ENFSI), the National Institute of Standards and Technology (NIST), the International Society for Forensic Genetics (ISFG), and the Combined DNA Index System (CODIS).

Kawsar Biotech Company offers several HID kits for various purposes. These kits include:

• RizFiling™ kit (with 12 markers along with the AMXY marker in a 5-color system) which has smaller fragment sizes suitable for degraded DNA, old samples, and bone DNA.
• IRFiling™ kit (with 17 markers in a 5-color system) is a preferred kit for most forensic medical cases. According to CODIS, a minimum of 13 markers is recommended for identity determination, and this kit uses 17 markers.
• IRFiling™ Globe (with 27 markers in a 6-color system) is currently used more for forensic medical purposes worldwide, especially when the FBI introduced new CODIS markers in 2016.
• The X chromosome-related kit called XFiling™ with 17 markers, 15 of which are specific to the X chromosome, operates in a 5-color system.
• The Y chromosome-related kit named YFiling™ (29-Plex) with 30 markers, also in a 5-color system, can be used for criminal investigations as well as male kinship analysis.

A comparison table has been prepared for selecting the appropriate human profiling kit for forensic medical purposes and identification in line with the goals of our dear customers.

DNA contains unique sequences of short tandem repeats (STRs) consisting of 3 to 7 base pairs scattered throughout the genome, which can be used for individual identification in forensic medicine. Since the exact number of repeats at each specific location varies among different individuals, forensic medicine can utilize these markers for identification purposes. Furthermore, each individual has two sets of STRs inherited from both parents, and each of these sets can have a different number of repeats at the same locus. In fact, a DNA profile is recognized as a DNA fingerprint that distinguishes individuals from each other and is only similar in identical twins.

The ™IRFiling v2.2 Kit is based on 17 markers, including 16 highly heterozygous STR markers and one gender determination marker. 15 of these markers are designed based on CODIS loci (CSF1PO, FGA, VWA, TH01, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, D2S1338, TPOX, and D19S433). Additionally, 7 of these 15 markers (marked with a solid line) also comply with the ESS or European standard, which ensures that the results obtained from this kit are internationally compliant and can provide responses with close to 100% accuracy in the shortest possible time. The SE33 marker is also included in the kit to enhance precision and discriminatory power. All 17 markers are amplified using the Multiplex PCR method, and their results are determined using capillary electrophoresis. The applications of the ™IRFiling v2.2 Kit include:

• Determination of kinship
• Human identity determination in accidents
• Criminal investigations
• Paternity and maternity testing
• Forensic genetics
• Determination of human profiles or patterns
• Population genetics and database creation

This kit can be used on DNA samples extracted from blood, amniotic fluid (AF), chorionic villus sampling (CVS), and DBC card (DNA storage card produced by KBC), blood lysing buffer (BLB), amniotic fluid lysing buffer (AFL), and chorionic villus lysing buffer (CVL).

For more information about the product, its usage, and result analysis, please refer to the user manual.