HBA Gap Extra Kit

HBA Gap Extra Kit – Detection of Common Alpha-Globin Gene Deletions

HBA Gap Extra Kit is a molecular diagnostic kit based on Gap-PCR, designed to aid in the detection of common alpha-globin gene deletions associated with alpha-thalassemia. Alpha-globin gene disorders can lead to different clinical conditions, ranging from mild forms of anemia to HbH disease and severe forms such as hydrops fetalis. These disorders are particularly prevalent in Southeast Asia, the Middle East, and Mediterranean populations.

The kit enables the detection of common alpha-globin gene deletions, including −α3.7, −α4.2, −−MED, and −−20.5 kb, as well as alpha-globin 3.7 triplication (anti-3.7) and even quadruplication. Detection of these alterations can be particularly useful in the molecular investigation of alpha-thalassemia and in distinguishing alpha-thalassemia from beta-thalassemia in relevant screening settings.

PCR products are analyzed using agarose gel electrophoresis. To facilitate result interpretation, the kit includes an HBA Gap Extra Allelic Ladder containing reference fragments for the normal alpha-globin gene, common deletions (3.7 kb, 4.2 kb, 20.5 kb, and MED), and anti-3.7 triplication, allowing direct comparison with unknown samples.

HBA Gap Extra Kit provides a straightforward agarose gel-based approach for the molecular detection of common alpha-globin gene deletions and copy-number alterations associated with alpha-thalassemia.

HBA Gap Extra Kit is a Gap-PCR kit for detecting common alpha-globin gene deletions, including 3.7 kb, 4.2 kb, 20.5 kb and MED, as well as alpha-globin 3.7 triplication, using agarose gel electrophoresis