
DNA Controls
According to the importance of prenatal diagnosis in molecular genetics and the possession of wide category of disease-causing mutations for different types of disease through years of prenatal diagnosis in human genetics laboratory of Dr. Zeinali, Kawsar Biotech Company has decided to offer positive DNA controls for PCR purposes.
A positive control may be necessary, when amplifying a new target sequence to confirm whether the primer set or primer–probe set works it can also be an absolute standard, which is a nucleic acid template of known quantitative information and absolute standard and used only to test for the presence or absence of a target. These DNAs have been extracted from immortalized lymphocytes with EB viruses.
Human genomic control DNA for PKU- PAH gene | |||
Name | Cat. No | Phenotype | Genotype |
KBC Normal DNA | K5200 | Homozygote Normal | Normal/Normal |
KBC mutant DNA | K5201 | Heterozygote | IVS10-11 G>A / N |
KBC mutant DNA | K5202 | Heterozygote | IVS2+5 (G>C) / N |
KBC mutant DNA | K5203 | Heterozygote | Cd 281 CCT>CTT(Pro>Leu) / N |
KBC mutant DNA | K5204 | Heterozygote | Cd 300 GCC>TCC (Ala>Ser) / N |
KBC mutant DNA | K5205 | Heterozygote | IVS 4+1 (G>C) /N |
KBC mutant DNA | K5206 | Heterozygote | IVS 9+5(G>A) /N |
KBC mutant DNA | K5207 | Heterozygote | Cd 261 CGA>CAA (Arg>Gln) /N |
KBC mutant DNA | K5208 | Heterozygote | IVS 4+5(G>T) / N |
KBC mutant DNA | K5209 | Heterozygote | IVS 11+1 (G>C)/N |
KBC mutant DNA | K5210 | Heterozygote | Cd 48 TTG>TCG(Leu>Ser) / N |
KBC mutant DNA | K5211 | Heterozygote | Cd 204 TAT>TGT (Tyr>Cys) / N or IVS6-96 A>G/N |
KBC mutant DNA | K5212 | Homozygote | IVS 10-11 G>A / IVS10-11 G>A |
KBC mutant DNA | K5213 | Homozygote | Cd390 GAG>GGG(Glu>Gly)/Cd390 GAG>GGG(Glu>Gly) |
KBC mutant DNA | K5214 | Homozygote | IVS 2+5(G>C) / IVS2+5(G>C) |
KBC mutant DNA | K5215 | Homozygote | Cd 261CGA>CAA (Arg>Gln) / Cd 261CGA>CAA (Arg>Gln) |
KBC mutant DNA | K5216 | Homozygote | IVS 4+5(G>T) / IVS 4+5(G>T) |
KBC mutant DNA | K5217 | Homozygote | Cd 261(CGA>TGA Arg>Stop)/ Cd 261(CGA>TGA Arg>Stop) |
KBC mutant DNA | K5218 | Compound heterozygote | Cd 252 CGG>TGG(Arg>Trp) /IVS2+5(G>C) |
KBC mutant DNA | K5219 | Homozygote | Cd 204 TAT>TGT/ Cd 204 TAT >TGT |
KBC mutant DNA | K5220 | Heterozygote | Cd 243 CGA>TGA /N |