KBC Max Pure Agarose

 | Post date: 2017/01/6 | 


Agarose is a neutral polysaccharide extracted from the cell walls of certain Rhodophyceae algae, also known as agarophyte seaweeds. Its chemical structure gives agarose the capacity to form very strong gels even at low concentrations. These gels have a macroreticular structure with a very open mesh which can be adjusted simply by varying the concentration of agarose.

The macroreticule of the agarose gel is formed by hydrogen bonds, which makes the gel thermo-reversible, thus it melts upon heating. The hysteresis – difference between gelling and melting temperature – is greater than in any other hydrocolloid. In addition, the absence of ionic groups makes the gel a neutral structure, thus avoiding interactions with hydrophilic macromolecules which migrate through the gel mesh.

All applications for agarose take advantage of the special characteristics of the macroreticular gel. It is used as a sieve or a support through which biological macromolecules such as proteins or nucleic acids can pass. Larger particles, such as viruses and subcellular fragments, are also able to move through the gel network.

ElectrophoresisAgarose is suitable for the widest range of electrophoretic procedures as well as in immunoelectrophoresis and electrofocusing. Driven by electrostatic fields, the macromolecules migrate through the macroreticular structure.

Features Agarose:

- High separation properties and sharp band patterns

- Easy solubility without foaming

- Excellent optical transparency

Applications Agarose:

-  Separation of PCR products

-  DNA: approx. 0.05 kbp – 50 kbp

-  RNA: approx. 0.30 kb – 20 kb

Specifications Agarose:

-  Gelling point (1.5%):                ≤ 37 °C

-  Melting point (1.5%):               ≤ 90 °C

-  Gel strength (1 %):                 1.175 g / cm2

-  Sulphate content:                    ≤ 0.10 % 

-  Moisture:                                ≤ 8.0 %

-  Clarity (1.5%) N.T.U:                3.73

-  DNA resolution:                       Passes test

- Dnase and Rnase activity:         None detected

Storage: Agarose can be stored at room temperature for more than 4 years






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KBC MaxPure Agarose


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