KBC DNA Purification Kit

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KBC PCR Purification kit is a silica base purification system. In this system, DNA of interest can be isolated by virtue of its ability to bind to silica membrane in the presence of high concentrations of chaotropic salts. These salts are then removed with an alcohol-based wash and the DNA is eluted in a low-ionic-strength solution such as TE buffer or dH2O.

The binding of DNA to silica seems to be driven by dehydration and hydrogen bond formation, which competes against weak electrostatic repulsion. Hence, a high concentration of salt will help drive DNA adsorption onto silica, and a low concentration will release the DNA.

KBC PCR Purification kit offers flexible centrifuge or vacuum-based options for purifying DNA fragments from gels or PCR reactions.




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