Write your message

KBC IRFiling Kit

 | Post date: 2017/05/21 | 




AWT IMAGE

 IRFiling™ Kit: DNA typing for human profiling and identification



In modern forensics, scientists use13 to 24 short tandem repeats (STRs) markers for forensic investigations, paternity indexing, etc. To create unique DNA profiles for different purposes. Our new human profiling kit with 17 loci, provides fast and accurate profile which is compatible with Iranian gene pool.
DNA extracted from biologic samples can be used as the genomic material in this method. In a multiplex assay specific STR markers are amplified and results are examined via capillary electrophoresis such as ABI 3500 Genetic analyzer. 





The KBC IRFiling STR Kit considers 16 markers used in AmpFlSTR Identifiler kit (Life Technologies, ABI, USA) namely D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, Amelogenin, D5S818, FGA. In addition SE33 STR marker which has been shown to have high heterozygosity in Iranian population. This locus is used in NGM Select and GlobalFiler kits. New primers sets have been designed and labeled with fluorescent dyes to be optimized for multiplex PCR. Size and fluorescent dye of all markers were designed to be completely different to aid international marketing.
To make a suitable ladder for the newly invented kit, DNA profile data of 10,000 individual were used. Different alleles were synthesized by their DNA that was extracted from DBC cards. Then the alleles were mixed together to make a ladder with most efficiency.
DNA profiles of 200 individuals were compared with the profile of ABI kit.  Both of them had the same result.





Since some more famous kits (e.g. from Thermo Fisher, US) have been used in Legal Medicine Org (LMO) and Police Dep’t, therefore we used same markers in this kit in addition to SE33 to improve its power of discrimination. Kit performance was as expected and was acceptable enough to compete with imported ones.


Additional Information
Company
Price
Amount
Description
Cat-No.

KBC IRFiling Kit Quick Protocol

KBC IRFiling Kit Panels
Kawsar Biotech Co
Contact us
50RXN


KBC IRFiling Kit
K3010-50
Kawsar Biotech Co
Contact us
100RXN
KBC IRFiling Kit
K3010-100

  

  ​

AWT IMAGE

IRFiling Allelic Ladder:

AWT IMAGE

IRFiling result:

AWT IMAGE

Comparing Identifiler with IRFiling kit:

AWT IMAGE



Warning: file_get_contents(./files/site1/modern01/tail_links_en.html): failed to open stream: No such file or directory in /home/kawsarbi/public_html/includes/template_code.php on line 121