RPMI

 | Post date: 2017/01/19 | 

Description

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. KBC offers a variety of RPMI 1640 Medium modifications for a range of cell culture applications.

Using RPMI

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

VOLUME

Sodium bicarbonate

sodium pyruvate

Glutamax

HEPES

Cell culture media

500 & 1000 ml

+

-

-

-

RPMI 1640

500 & 1000 ml

+

-

-

+

RPMI 1640, HEPES

500 & 1000 ml

+

-

+

+

RPMI 1640, HEPES,Glutamax

500 & 1000 ml

+

+

-

+

RPMI 1640, HEPES, sodium pyruvate

500 & 1000 ml

+

+

+

+

RPMI 1640, HEPES, sodium pyruvate,Glutamax

500 & 1000 ml

+

+

+

-

RPMI 1640, sodium pyruvate,Glutamax




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